*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products. (PubMed link indicates BioLegend citation) It is recommended that the reagent be titrated for optimal performance for each application.Īdditional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections 3-6, Western blotting 2, immunoprecipitation 1,2, immunofluorescence 7,8, and ELISA 9.Ĭlone M3/38 has been reported to recognize residues 48-100 in the amino-terminal domain of galectin-3 7. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10 6 cells in 100 µL volume or 100 µL of whole blood. IHC, ELISA - Reported in the literature, not verified in houseĮach lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The antibody solution should be stored undiluted between 2☌ and 8☌. The antibody was purified by affinity chromatography. Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Raised against galectin-3 of mouse origin Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control. Proteins were visualized using an HRP Goat anti-rat IgG Antibody and chemiluminescence detection. Total cell lysate from MCF7 cells (lane 1, 15 µg), Jurkat cells (lane 2, 15 µg), 3T3-L1 (lane 3, 15 µg) and Raw264.7 (lane 4, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with purified anti-mouse/human Mac-2 (Galectin-3) antibody (clone M3/38). Western blot analysis was performed using anti‐mouse/human Mac‐2 antibody (clone Gal397). Lane 1 was immunoprecipitated with a control antibody and lane 2 was immunoprecipitated with anti‐mouse/human Mac‐2 (Galectin‐3) Antibody (clone M3/38). 150 µg of HeLa cell lysates were tested at a protein concentration of 1 µg/µL for each sample. Immunoprecipitation/Western Blot analysis using anti‐mouse/human Mac‐2 (Galectin‐3) Antibody (clone M3/38). HeLa cells were stained with purified anti-Galectin 3 (M3/38) antibody, followed by staining with DyLight™ 594 conjugated goat anti-mouse IgG (red) antibody. BALB/c peritoneal macrophages stained with M3/38 purified, followed by anti-rat IgG FITC
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